1UV0
Pancreatitis-associated protein 1 from human
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW21B |
| Synchrotron site | LURE |
| Beamline | DW21B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.730, 49.350, 92.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.750 - 1.780 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lit |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.750 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.082 | 0.317 |
| Number of reflections | 11726 | |
| <I/σ(I)> | 6.3 | 2.4 |
| Completeness [%] | 84.3 | 74.6 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 1 MICROL PROTEIN 3.0 MG/ML, IN 10MM SODIUM ACETATE BUFFER, 0.5 MICROL MOTHER LIQUOR: 0.1M SODIUM CACODYLATE PH6.0, PEG 8000 20% (W/V), 0.2M ZINC ACETATE, 5% GLYCEROL, RESERVOIR 1ML, pH 4.50 |
