1UUT
The Nuclease Domain of Adeno-Associated Virus Rep Complexed with the RBE' Stemloop of the Viral Inverted Terminal Repeat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.830, 78.705, 184.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.25700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m55 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.350 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.161 |
| Total number of observations | 158265 * | |
| Number of reflections | 38009 | |
| <I/σ(I)> | 19.7 | 3.8 |
| Completeness [%] | 89.7 * | 57 |
| Redundancy | 4.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 20 (mM) | pH7.5 |
| 2 | 1 | reservoir | 0.12 (M) | ||
| 3 | 1 | drop | magnesium acetate | 0.2 (M) | |
| 4 | 1 | drop | cacodylate | 0.1 (M) | pH6.5 |
| 5 | 1 | drop | PEG8000 | 20 (%(w/v)) |
