1UUS
Structure of an activated Dictyostelium STAT in its DNA-unbound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 125.900, 145.000, 66.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.800 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25600 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.073 | 0.354 * |
| Number of reflections | 15362 | |
| <I/σ(I)> | 13.4 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 1 M NaCl, 150 mM ammonium acetate, 5 mM MgSO4, 50 mM MES pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 250 (mM) | ||
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 3 | 1 | drop | PMSF | 0.2 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | protein | 6-7 (mg/ml) | |
| 6 | 1 | reservoir | 1 (M) | ||
| 7 | 1 | reservoir | ammonium acetate | 150 (mM) | |
| 8 | 1 | reservoir | 5 (mM) | ||
| 9 | 1 | reservoir | MES | 50 (mM) | pH6. |
