1UUS

Structure of an activated Dictyostelium STAT in its DNA-unbound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	C 2 2 21
Unit cell lengths	125.900, 145.000, 66.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.800
R-factor	0.198
Rwork	0.198
R-free	0.25600 *
Structure solution method	MIRAS
RMSD bond length	0.009 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.073	0.354 *
Number of reflections	15362
<I/σ(I)>	13.4	3
Completeness [%]	99.8	100
Redundancy	4.4	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *		1 M NaCl, 150 mM ammonium acetate, 5 mM MgSO4, 50 mM MES pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		250 (mM)
2	1	drop	HEPES	20 (mM)	pH7.5
3	1	drop	PMSF	0.2 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	drop	protein	6-7 (mg/ml)
6	1	reservoir		1 (M)
7	1	reservoir	ammonium acetate	150 (mM)
8	1	reservoir		5 (mM)
9	1	reservoir	MES	50 (mM)	pH6.