1UU8

Structure of human PDK1 kinase domain in complex with BIM-1

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2.5 Å)

Cell axes122.397122.39748.016
Cell angles90.0090.00120.00
SpacegroupP 32 2 1
Resolution limits24.32 - 2.50
the highest resolution shell value -
R-factor0.17642
R-work0.19800 (0.19900*)
R-free0.25800 (0.25900*)
RMSD bond length0.010*
RMSD bond angle1.720*

Data Collection Statistics

Resolution limits25.00 - 2.50
the highest resolution shell value -
Number of reflections13556
Number of measurements40461*
Rmerge_l_obs0.089
the highest resolution shell value0.549
Completeness92.6
the highest resolution shell value75.6*
Redundancy3 (3.0*)
the highest resolution shell value2.6

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1Vapor diffusion, sitting drop*7.5K*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirammonium sulfate2.1 (M)
21reservoirTris-HCl0.1 (M)pH7.5
31dropethanol10 (mM)
41dropprotein6.6 (mg/ml)
Annotated Information is extracted from Literature Info*