1UTY
Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 102.296, 102.296, 77.915 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.265 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.059 | 0.365 |
Number of reflections | 18244 | |
<I/σ(I)> | 34.57 | 6.6 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 20% JEFFAMINE M-600 PH 7.6, 0.65 M NACL, 10 MM SODIUM PHOSPHATE |