1UTX
Regulation of Cytolysin Expression by Enterococcus faecalis: Role of CylR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS M18X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 41 |
| Unit cell lengths | 63.679, 63.679, 41.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.900 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.19300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.104 | 0.440 |
| Number of reflections | 13086 | |
| <I/σ(I)> | 19 | 5.8 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 14.8 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | SALTING-IN BY DIALYSIS AGAINST 0.2 M NAI, 10 % GLYCEROL, 50 MM HEPES PH 7.0 |
