1UTI
Mona/Gads SH3C in complex with HPK derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.456, 27.442, 33.043 |
| Unit cell angles | 90.00, 104.22, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.500 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oeb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.113 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.030 | 0.065 |
| Number of reflections | 9855 | |
| <I/σ(I)> | 25.2 | 14.7 |
| Completeness [%] | 97.4 | 98.2 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2M AMMONIUM SULFATE, 0.05M HEPES PH7.5, pH 7.50 |
