1UT5
Divalent metal ions (manganese) bound to T5 5'-exonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-11-15 |
| Detector | XENTRONICS |
| Spacegroup name | P 43 |
| Unit cell lengths | 77.600, 77.600, 134.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.750 |
| Rwork | 0.252 |
| R-free | 0.33400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.750 |
| Rmerge | 0.041 |
| Total number of observations | 19024 * |
| Number of reflections | 16150 * |
| Completeness [%] | 74.6 |
| Redundancy | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Ceska, T.A., (1993) J.Mol.Biol., 233, 179. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 35 (%(w/v)) | |
| 4 | 1 | drop | potassium phosphate | 25 (mM) | pH7.5 |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | 100 (mM) | ||
| 8 | 1 | drop | glycerol | 5 (%(v/v)) |
