1UT4

Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	61.953, 75.224, 80.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.228
Rwork	0.225
R-free	0.28100 *
Structure solution method	MIRAS
RMSD bond length	0.015
RMSD bond angle	1.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.094	0.300
Number of reflections	11476
<I/σ(I)>	5.6	2.5
Completeness [%]	95.6	89.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		Olsen, A., (2004) Acta Crystallogr.,Sect.D, 60, 112. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10-15 (%)
2	1	reservoir	imidazole-malic acid	0.1 (M)	pH7.0
3	1	drop	protein	3.2-7.7 (mg/ml)