1USX
Crystal structure of the Newcastle disease virus hemagglutinin-neuraminidase complexed with thiosialoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-15 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 115.070, 115.070, 283.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.700 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e8v |
RMSD bond length | 0.007 |
RMSD bond angle | 1.460 |
Data reduction software | MOSFLM |
Phasing software | CCP4 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.079 * | 0.254 * |
Total number of observations | 454555 * | |
Number of reflections | 39099 * | |
<I/σ(I)> | 8.9 | 2 |
Completeness [%] | 90.4 * | 88.4 * |
Redundancy | 10.2 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.3 * | pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | thiosialoside | 100 (mM) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | PEG3350 | 20 (%) | |
5 | 1 | reservoir | HEPES | 0.1 (M) | pH6.3 |
6 | 1 | reservoir | 2 (mM) |