1USQ
Complex of E. Coli DraE adhesin with Chloramphenicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 |
| Unit cell lengths | 118.969, 118.969, 57.416 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.847 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.330 |
| Number of reflections | 71543 | |
| <I/σ(I)> | 4.2 | 1.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.7 M AMMONIUM SULPHATE 0.1M TRIS-HCL PH 7.0, 2.8 MM CHLORAMPHENICOL |
