1USO
DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR, PRO9LEU MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-15 |
Detector | RIGAKU IMAGE PLATE RAXIS-V |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.430, 48.687, 94.021 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.250 - 1.300 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1usm |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.037 | 0.083 |
Number of reflections | 34147 | |
<I/σ(I)> | 29.35 | 17 |
Completeness [%] | 94.0 | 95 |
Redundancy | 4.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | VAPOR DIFFUSION METHOD, RESERVOIR SOLUTION 15% PEG8000 AND 0.2M AMMONIUM SULFATE. CRYOPROTECTANT 22.5% GLYCEROL, 15% PEG8000 AND 0.2M AMMONIUM SULFATE, pH 6.00 |