1USM
DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR, PRO9LEU MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-02-15 |
Detector | RIGAKU IMAGE PLATE RAXIS-V |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 55.625, 55.625, 125.882 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.770 - 1.200 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dco |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.034 | 0.146 |
Number of reflections | 30991 | |
<I/σ(I)> | 41.4 | 11.7 |
Completeness [%] | 98.7 | 99.5 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.3 | MICROBATCH METHOD UNDER OIL BY CRYSTALLIZATION ROBOT TERA. 25.6 MG/ML OF PROTEIN SOLUTION WAS MIXED WITH 3.15M SODIUM FORMATE AND 0.1M SODIUM ACETATE BUFFER, PH 4.0. FINAL PH WAS 5.3. CRYOPROTECTANT CONTENT WAS: 25% GLYCEROL, 2.0M SODIUM FORMATE, AND 0.6M SODIUM ACETATE BUFFER, PH 4.0. |