1USE
human VASP tetramerisation domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2001-12-15 |
| Spacegroup name | I 4 |
| Unit cell lengths | 29.701, 29.701, 100.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.300 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.028 | 0.279 |
| Number of reflections | 95581 | |
| Completeness [%] | 98.6 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 35% PEG400, 0.2M SODIUM CITRATE, 0.1M TRIS PH8.5, pH 8.50 |
