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human VASP tetramerisation domain L352M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Collection date	2002-05-15
Spacegroup name	I 4
Unit cell lengths	29.648, 29.648, 100.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.212
Rwork	0.211
R-free	0.22400
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.035	0.309
Number of reflections	54657
Completeness [%]	99.8	99.9
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		25% PEG MME550, 0.2M NA-CITRATE, 0.1M TRIS PH8.5, pH 8.50