1USC
PUTATIVE STYRENE MONOOXYGENASE SMALL COMPONENT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-02-15 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.321, 77.571, 63.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.990 - 1.240 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.22200 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.260 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.044 | 0.282 |
Number of reflections | 96245 | |
<I/σ(I)> | 28.95 | 2.8 |
Completeness [%] | 97.0 | 94.8 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.7 | 291 | VAPOR DIFFUSION METHOD, 3.1 MG/ML OF PROTEIN SOLUTION WAS MIXED WITH RESERVOIR SOLUTION (21% PEG4000, 0.1M SODIUM ACETATE PH 4.7) AND EQUILIBRATED AGAINST RESERVOIR SOLUTION AT ROOM TEMPERATURE (291 K). CRYOPROTECTANT: 18% GLYCEROL, 24% PEG4000, 0.1M SODIUM ACETATE PH 4.7 |