1US8
The Rad50 signature motif: essential to ATP binding and biological function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 294 |
| Collection date | 2001-11-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.541, 67.851, 69.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.100 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f2t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.140 |
| High resolution limit [Å] | 2.100 | 2.050 |
| Rmerge | 0.057 | 0.279 |
| Total number of observations | 200189 * | |
| Number of reflections | 25989 | |
| <I/σ(I)> | 10 | 3 |
| Completeness [%] | 99.2 | 98.9 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 20 % PEG8000, 0.1 M MES PH 6.0, 0.2 M CALCIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 10 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | drop | ATPgammaS | 2.5 (mM) | |
| 7 | 1 | drop | 10 (mM) | ||
| 8 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 9 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
