1URZ
Low pH induced, membrane fusion conformation of the envelope protein of tick-borne encephalitis virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 121.500, 142.900, 173.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.700 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1svb |
RMSD bond length | 0.007 |
RMSD bond angle | 25.900 * |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.111 | 0.455 |
Total number of observations | 306381 * | |
Number of reflections | 80896 | |
<I/σ(I)> | 5.4 | 2 |
Completeness [%] | 96.9 | 83.9 |
Redundancy | 3.8 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | Stiasny, K., (2004) J. Virol., 78, 3178. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2-5 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
3 | 1 | reservoir | PEG2000 | 20-30 (%) |