1URX
Crystallographic structure of beta-agarase A in complex with oligoagarose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 193 |
| Collection date | 2003-03-15 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 51.380, 51.380, 205.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.200 - 1.700 |
| R-factor | 0.15642 |
| Rwork | 0.155 |
| R-free | 0.18200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o4y |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.762 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 0.110 |
| Total number of observations | 148960 * | |
| Number of reflections | 34045 | |
| <I/σ(I)> | 12.1 | 6.8 |
| Completeness [%] | 95.3 | 98.4 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20.5 * | 30 % PEG 4000 200 MM AMMONIUM ACETATE, 100 MM SODIUM ACETATE PH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | agaro-octaose | 4 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 7 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
