1URU
Amphiphysin BAR domain from Drosophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 49.586, 49.586, 190.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.250 - 2.600 |
| R-factor | 0.23 * |
| Rwork | 0.236 |
| R-free | 0.30636 |
| Structure solution method | OTHER |
| RMSD bond length | 0.013 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.940 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.134 | 0.010 * |
| Number of reflections | 8657 | |
| <I/σ(I)> | 4.9102 | 0.73 |
| Completeness [%] | 97.2 | 86 |
| Redundancy | 10.21 | 9.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 * | 2.5MG/ML PROTEIN SOLUTION IN 18% PEG4000, 200MM NACL, 1MM DTT, 20MM HEPES PH 7.4 MIXED WITH THE WELL SOLUTION 18% PEG 4000, 0.2M AMMONIUM ACETATE, 100MM SODIUM CITRATE PH 6.0. CRYSTALS WERE EQUILIBRATED IN 20% GLYCEROL FOR COOLING TO 100K. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 18 (%) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | HEPES | 20 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 18 (%) | |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | sodium citrate | 100 (mM) |
