1URT
MURINE CARBONIC ANHYDRASE V
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-02 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.090, 67.520, 48.860 |
| Unit cell angles | 90.00, 107.60, 90.00 |
Refinement procedure
| Resolution | 6.500 - 2.800 |
| R-factor | 0.137 |
| Rwork | 0.137 |
| R-free | 0.24100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.600 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.088 |
| Total number of observations | 19846 * |
| Number of reflections | 6760 * |
| Completeness [%] | 87.0 * |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | sucrose monolaurate | 2.0 (mM) | |
| 4 | 1 | drop | PEG8000 | 16-12 (%) | |
| 5 | 1 | drop | sodium acetate | 0.2 (M) | |
| 6 | 1 | drop | sodium cacodylate | 0.1 (M) | |
| 7 | 1 | reservoir | PEG8000 | 16-12 (%) | |
| 8 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 9 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
