1URO
UROPORPHYRINOGEN DECARBOXYLASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9252, 0.9795, 0.9798, 1.0688 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.000, 103.000, 73.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.049 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.176 |
| Total number of observations | 156721 * | |
| Number of reflections | 42721 | |
| <I/σ(I)> | 15 | 3.5 |
| Completeness [%] | 96.2 | 91.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 4 * | CITRATE BUFFER, pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 36 (mM) | pH7.5 |
| 3 | 1 | drop | beta-mercaptoethanol | 0.7 (mM) | |
| 4 | 1 | drop | glycerol | 7 (%) | |
| 5 | 1 | drop | citrate | 0.3-0.5 (M) | |
| 6 | 1 | reservoir | citrate | 1.2-1.7 (M) | pH5.8-6.2 |
