1URJ
Single stranded DNA-binding protein(ICP8) from Herpes simplex virus-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.910, 145.370, 162.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.28570 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 20.000 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 47265 | |
| <I/σ(I)> | 9.8 | 2.98 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 12% PEG 3K, 0.1 M SODIUM/POTASSIUM PHASPHATE PH 6.0 |
