1URB

ALKALINE PHOSPHATASE (N51MG)

Experimental procedure

Collection date	1994-09-28
Spacegroup name	I 2 2 2
Unit cell lengths	195.720, 168.480, 76.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.140
R-factor	0.193
Rwork	0.193
R-free	0.24100
RMSD bond length	0.015
RMSD bond angle	23.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.140 *
Rmerge	0.086 *
Total number of observations	186425 *
Number of reflections	61905
Completeness [%]	88.3
Redundancy	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *		Chen, L. C., (1992) Protein Eng., 5, 605. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		20 (%(w/v))
2	1	drop	Tris	100 (mM)
3	1	drop		10 (mM)
4	1	drop		0.01 (mM)
5	1	drop	protain	30 (mg/ml)
6	1	reservoir		31.6 (%(w/v))
7	1	reservoir	Tris	100 (mM)
8	1	reservoir		10 (mM)