1UR4
The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-02-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.330, 79.520, 104.890 |
Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.200 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.098 | 0.374 |
Number of reflections | 40969 | |
Completeness [%] | 98.8 | 96.5 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 26% PEG 1500, pH 5.00 |