1UR2
Xylanase Xyn10B mutant (E262S) from Cellvibrio mixtus in complex with arabinofuranose alpha 1,3 linked to xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.924, 67.659, 104.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.600 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uqy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.319 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.432 |
| Number of reflections | 42050 | |
| <I/σ(I)> | 1.93 | 1.93 |
| Completeness [%] | 98.3 | 90.3 |
| Redundancy | 3.25 | 2.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 18 * | 0.2M MGCL2 0.1M TRIS HCL PH8.5, 30% PEG 4K, 5% PEG 400., pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | xylooligosaccharide | 10 (mM) | |
| 3 | 1 | reservoir | 0.20 (M) | ||
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | PEG400 | 5 (%) |
