1UQW
Crystal structure of yliB protein from escherichia coi
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98025,0.98051,0.96 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 152.560, 82.540, 93.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.720 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.077 | 0.207 |
| Number of reflections | 32994 | |
| <I/σ(I)> | 6.2 | 3.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PEG 8000 15%, ZINC ACETATE 0.2M, SODIUM ACETATE 0.1M PH6.0, GLYCEROL 5%, pH 6.00 |
