1UQS
The Crystal Structure of Human CD1b with a Bound Bacterial Glycolipid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.974, 96.974, 114.834 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 3.100 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.29100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzq |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 2.110 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.077 | 0.604 |
| Total number of observations | 150075 * | |
| Number of reflections | 11697 | |
| <I/σ(I)> | 34.9 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 * | 20 * | 0.1M SODIUM CITRATE PH 5.6, 0.5M AMMONIUM SULFATE, 0.5M LITHIUM SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Tris-Cl | 20 (mM) | pH6.5 |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 6 | 1 | reservoir | ammonium sulfate | 0.20-1.25 (M) | |
| 7 | 1 | reservoir | 0.20-0.75 (M) |
