1UQ4
RICIN A-CHAIN (RECOMBINANT) R213D MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-17 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 67.300, 67.300, 140.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.900 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ift |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.133 |
| Total number of observations | 53233 * | |
| Number of reflections | 22494 * | |
| <I/σ(I)> | 21.6 | 4.7 |
| Completeness [%] | 85.6 * | 43.6 * |
| Redundancy | 2.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.9 * | 4 * | Weston, S.A., (1994) J. Mol. Biol., 244, 410. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.875 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 56.2 (mM) | |
| 3 | 1 | drop | EDTA | 0.75 (mM) | |
| 4 | 1 | drop | dithiothreitol | 3.75 (mM) | |
| 5 | 1 | drop | sodium citrate | 16.7 (mM) | |
| 6 | 1 | drop | ammonium sulfate | 10 (%(v/v)) | |
| 7 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 30 (%(v/v)) |
