1UPR
Crystal structure of the PEPP1 pleckstrin homology domain in complex with Inositol 1,3,4,5-tetrakisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-15 |
Detector | ADSC CCD |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 92.577, 92.577, 35.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.270 |
R-factor | 0.24 |
Rwork | 0.237 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1upq |
RMSD bond length | 0.012 |
RMSD bond angle | 1.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.055 | 0.329 |
Number of reflections | 7339 | |
<I/σ(I)> | 30.2 | 6.4 |
Completeness [%] | 96.7 | 96.2 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 16% PEG 20,000, 0.1 M MES (PH 6.0) |