1UPK
Crystal structure of MO25 in complex with a C-terminal peptide of STRAD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.979355,0.979333 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.650, 134.033, 40.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.850 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23100 * |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 18.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.476 |
| Total number of observations | 324698 * | |
| Number of reflections | 36794 | |
| <I/σ(I)> | 41.7 | 4.9 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 8.8 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 15% PEG 20000, 0.1 M MES PH 5.0, 3.3% W/V D-GLUCOSE MONOHYDRATE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG20000 | 15 (%(w/v)) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH5.0 |
| 4 | 1 | reservoir | D-glucose monohydrate | 30 (%(w/v)) |
