1UP8
Recombinant vanadium-dependent bromoperoxidase from red algae Corallina pilulifera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-15 |
Detector | ADSC CCD |
Spacegroup name | P 63 |
Unit cell lengths | 185.948, 185.948, 180.279 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.000 - 2.200 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qhb |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.123 | 0.403 |
Number of reflections | 178580 | |
<I/σ(I)> | 10.5 | 2.3 |
Completeness [%] | 98.5 | 98 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 3.8 * | 0.1M TRIS/H2SO4, 2M NH4H2PO4, pH 4.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 50 (mM) | pH7.4 |
2 | 1 | drop | citric acid-potassium dihydrogenphosphate | 100 (mM) | pH3.8 |
3 | 1 | drop | EDTA | 10 (mM) |