1UP5
Chicken Calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 1995-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.696, 53.073, 24.572 |
| Unit cell angles | 93.22, 96.86, 89.19 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clm |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.832 |
| Data reduction software | ADSC |
| Data scaling software | ADSC |
| Phasing software | EPMR (V. 2.5) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.460 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.032 | 0.410 |
| Number of reflections | 21890 | |
| <I/σ(I)> | 8.6 | 1.89 |
| Completeness [%] | 77.8 | 67.4 |
| Redundancy | 1.14 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | HANGING DROPS 12UL CAM 12MG/ML + 8UL PRECIPITANT, SEEDED 10MM NAOAC, PH 4.0, 30% MPD, 15% ISOPROPANOL |
