1UP4
Structure of the 6-phospho-beta glucosidase from Thermotoga maritima at 2.85 Angstrom resolution in the monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Collection date | 2002-11-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.048, 188.315, 125.628 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.850 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23500 |
| Structure solution method | OTHER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.371 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.110 | 0.382 |
| Number of reflections | 103065 | |
| <I/σ(I)> | 12.3 | 3.4 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 200 MM CALCIUM ACETATE, 100MM SODIUM ACETATE PH 4.6, 5% PEG 4000. PROTEIN AT 5 MG/ML |
