1UOZ
Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with thiocellopentaose at 1.1 angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2002-03-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.940, 92.627, 46.677 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.300 - 1.100 |
R-factor | 0.113 |
Rwork | 0.112 |
R-free | 0.13000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tml |
RMSD bond length | 0.015 |
RMSD bond angle | 1.762 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.200 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.075 | 0.123 |
Number of reflections | 109101 | |
<I/σ(I)> | 17 | 7.9 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 5.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 14 % PEG 4K, 100 MM HEPES 7.5, 200 MM LISO4. THE PROTEIN WAS AT 10MG/ML AND INCUBATED WITH 1 MM OF THIOCELLOPENTAOSE FOR 1HR PRIOR. CRYSTALLISATION. 20% GLYCEROL WAS ADDED AS CRYOPROTECTANT, pH 7.50 |