1UOR
X-RAY STUDY OF RECOMBINANT HUMAN SERUM ALBUMIN. PHASES DETERMINED BY MOLECULAR REPLACEMENT METHOD, USING LOW RESOLUTION STRUCTURE MODEL OF TETRAGONAL FORM OF HUMAN SERUM ALBUMIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.860, 88.280, 60.680 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN SERUM ALBUMIN (TETRAGONAL) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.270 |
| Rmerge | 0.080 |
| Number of reflections | 14772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 20 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 120-180 (mg/ml) | |
| 2 | 1 | drop | PEG400 | 40 (%) | |
| 3 | 1 | drop | 0.05 (M) | ||
| 4 | 1 | reservoir | 0.05 (M) | ||
| 5 | 1 | reservoir | PEG400 | 40 (%) |
