1UOH

HUMAN GANKYRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	120
Detector technology	CCD
Collection date	2003-02-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	37.606, 64.622, 74.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.440 - 2.000
R-factor	0.194
Rwork	0.191
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mj0
RMSD bond length	0.018
RMSD bond angle	1.658
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.040 *
Rmerge	0.076	0.583
Total number of observations	194568 *
Number of reflections	12976 *
<I/σ(I)>	10.13	1.79
Completeness [%]	95.6	88.6
Redundancy	2.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Krzywda, S., (2003) Acta Crystallogr., D59, 1294. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)	pH7.5
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	PEG5000 MME	24 (%(w/v))
4	1	reservoir	MES	0.1 (M)	pH6.4