1UOH
HUMAN GANKYRIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.606, 64.622, 74.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.440 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mj0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.658 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.040 * |
| Rmerge | 0.076 | 0.583 |
| Total number of observations | 194568 * | |
| Number of reflections | 12976 * | |
| <I/σ(I)> | 10.13 | 1.79 |
| Completeness [%] | 95.6 | 88.6 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Krzywda, S., (2003) Acta Crystallogr., D59, 1294. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | PEG5000 MME | 24 (%(w/v)) | |
| 4 | 1 | reservoir | MES | 0.1 (M) | pH6.4 |
