1UO3
Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 114 |
Collection date | 2003-02-15 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 79.195, 79.195, 79.195 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.900 - 1.920 |
R-factor | 0.2273 |
Rwork | 0.238 |
R-free | 0.28420 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.598 |
High resolution limit [Å] | 1.920 |
Rmerge | 0.059 |
Number of reflections | 6964 |
<I/σ(I)> | 7.5 |
Completeness [%] | 100.0 |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROP, RESERVOIR: 10%W/V PEG 6K, 2 M NACL, PH 7.0 |