1UNY
Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 114 |
| Collection date | 2002-10-15 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 79.348, 79.348, 79.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.900 - 2.300 |
| R-factor | 0.2273 |
| Rwork | 0.253 |
| R-free | 0.32202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.300 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.140 |
| Number of reflections | 4127 |
| <I/σ(I)> | 3.4 |
| Completeness [%] | 100.0 |
| Redundancy | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.5 M NACL, 100 MM NAAC, 200 MM LI2SO4, PH 4.5 |
