1UNP
Crystal structure of the pleckstrin homology domain of PKB alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.064, 33.803, 42.071 |
Unit cell angles | 90.00, 119.48, 90.00 |
Refinement procedure
Resolution | 19.780 - 1.650 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.23600 |
Structure solution method | MAD |
Starting model (for MR) | 1h10 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.048 | 0.409 |
Number of reflections | 12409 | |
<I/σ(I)> | 4.1 | 4.1 |
Completeness [%] | 98.7 | 97.2 |
Redundancy | 3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 30 % PEG 4000, 0.25 M SODIUM ACETATE, 0.1 M TRIS PH 8.5 3.3 % POLYPROPYLENE GLYCOL 400 |