1UNN

Complex of beta-clamp processivity factor and little finger domain of PolIV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	146.451, 70.117, 110.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.100 - 1.900
Rwork	0.179
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pol
RMSD bond length	0.032
RMSD bond angle	2.712
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.100 *	2.010
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.201
Number of reflections	90134	12565 *
<I/σ(I)>	8.3	3.5
Completeness [%]	96.5	87.4
Redundancy	4.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	14 *	CRYSTALS WERE OBTAINED USING HANGING DROP METHOD AT 14C. WELL CONSISTED OF 1.2M AMMONIUM SULPHATE, 50MM SODIUM ACETATE PH 4.6. 2UL DROP CONSISTED OF 1UL WELL SOLUTION PLUS 1UL PROTEIN SOLUTION (50MM HEPES PH7.0, 2MM MAGNESIUM CHLORIDE, 200MM SODIUM CHLORIDE AND PROTEIN AT 6MG/ML).

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	ammonium sulfate	1.2 (M)
3	1	reservoir	sodium acetate	50 (mM)	pH4.6