1UNA
UNASSEMBLED VIRUS COAT PROTEIN DIMER, BACTERIOPHAGE RNA-BINDING DIMER
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-12-03 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.300, 60.500, 67.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| Rwork | 0.204 |
| R-free | 0.28400 |
| Structure solution method | MOL. REPLACEMENT |
| Starting model (for MR) | MS2 COAT PROTEIN DIMER (1MS2) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.031 |
| Data reduction software | XENGEN ((HOWARD) |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 4.790 * | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 * | 0.123 * |
| Total number of observations | 32352 * | |
| Number of reflections | 6248 * | 1157 * |
| <I/σ(I)> | 12.9 | 5 |
| Completeness [%] | 94.0 | 81 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 13 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | GA dimers | 3 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | Tris-maleate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG1000 |
