1UN9
Crystal structure of the dihydroxyacetone kinase from C. freundii in complex with AMP-PNP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.788, 124.869, 236.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.100 |
R-factor | 0.237 |
Rwork | 0.237 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.180 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.086 | 0.310 |
Number of reflections | 25784 | |
<I/σ(I)> | 9.3 | 6.5 |
Completeness [%] | 87.4 | 86 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |