1UN8
Crystal structure of the dihydroxyacetone kinase of C. freundii (native form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.440, 124.590, 237.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.500 |
| R-factor | 0.2 |
| Rwork | 0.196 |
| R-free | 0.24500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.432 * |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.066 | 0.361 * |
| Number of reflections | 50187 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 96.8 | 83.7 * |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30-50 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.3 |
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | PEG3000 | 9 (%(w/v)) | |
| 7 | 1 | reservoir | glycerol | 25 (%(v/v)) |
