1UN1

Xyloglucan endotransglycosylase native structure.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Detector	ADSC CCD
Spacegroup name	P 63
Unit cell lengths	188.762, 188.762, 45.973
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.800 * - 2.100
R-factor	0.208
Rwork	0.206
R-free	0.23400 *
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.480 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RSPS
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.800 *	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.261 *
Number of reflections	106310
<I/σ(I)>	12.4	3.7
Completeness [%]	99.4	95.5
Redundancy	3.12	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	293 *	10 MG ML-1 PROTEIN SOLUTION 1:1 WITH 1.0 M NAOAC AND 0.2 M IMIDAZOLE PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		1.0 (M)
3	1	reservoir	imidazole	0.2 (M)	pH6.5