1UN0
Crystal Structure of Yeast Karyopherin (Importin) alpha in complex with a Nup2p N-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.813, 140.076, 63.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.21781 |
| Rwork | 0.216 |
| R-free | 0.25700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ee4 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.831 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.086 | 0.531 |
| Total number of observations | 76810 * | |
| Number of reflections | 29670 | 4308 * |
| <I/σ(I)> | 10 | 1.8 |
| Completeness [%] | 97.5 | 98.3 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | 18 * | 50 MM HEPES PH 6.8, 0.15 M NACL, 24 % PEG3350, 2 % PEG400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | pH6.8 |
| 3 | 1 | drop | 0.15 (M) | ||
| 4 | 1 | drop | PEG3350 | 24 (%) | |
| 5 | 1 | drop | PEG400 | 2 (%) |
