1UMW
Structure of a human Plk1 Polo-box domain/phosphopeptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.352, 79.518, 61.993 |
| Unit cell angles | 90.00, 93.26, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.229 |
| Rwork | 0.230 |
| R-free | 0.25800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.900 * | 1.850 |
| Rmerge | 0.053 | 0.530 |
| Number of reflections | 50058 | |
| <I/σ(I)> | 20 | 2.6 |
| Completeness [%] | 97.7 | 97.1 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 18 * | pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | peptide | 0.2 (mM) | |
| 2 | 1 | 1 | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | 1 | 500 (mM) | ||
| 4 | 1 | 1 | EDTA | 1 (mM) | |
| 5 | 1 | 1 | dithiothreitol | 3 (mM) |
