1UMI
Structural basis of sugar-recognizing ubiquitin ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 63.806, 63.806, 147.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.720 - 2.400 |
R-factor | 0.19968 |
Rwork | 0.197 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.043 |
RMSD bond angle | 3.319 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.720 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 11950 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 7.5 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium chloride, ammonium sulphate, PIPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |