1UMG
Crystal structure of fructose-1,6-bisphosphatase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9712, 0.9792, 0.9794 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 111.778, 111.778, 153.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.230 - 1.800 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.19700 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 44654 | |
<I/σ(I)> | 27.7 | 8.9 |
Completeness [%] | 99.1 | 99.7 |
Redundancy | 3.22 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.2 | 298 | PEG 8000, magnesium chloride, sodium chloride, Tris-HCl, fructose-1,6-bisphosphate, pH 8.2, MICROBATCH, temperature 298.0K |