1UMF
crystal structure of chorismate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-10 |
Detector | MACSCIENCE |
Wavelength(s) | 1 |
Spacegroup name | I 4 |
Unit cell lengths | 146.478, 146.478, 132.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.130 - 2.250 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.390 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 64913 | |
Completeness [%] | 98.5 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | PEG 400, CaCl2, HEPES, DTT, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |